The Vile Matron is the Boss of the Biotics Lab stage in Killing Floor: Incursion and the final boss of the game.

It is a large creature capable of creating zeds (possibly an evolution of the Clot Mother), and the Patriarch wants the data on how to develop them.

The Vile Matron is fought in several phases, as the player activates elevators to rise higher and continue the fight.

Ultimately, the player must decide to either give the data to the Patriarch to save the people he's taken hostage, or give the data to Mr. Foster to ensure the Patriarch won't be able to use it.